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methylation sensitive restriction enzyme apeki  (New England Biolabs)


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    New England Biolabs methylation sensitive restriction enzyme apeki
    Methylation Sensitive Restriction Enzyme Apeki, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methyl+sensitive+enzyme/ApeKI/pm42080968-293-7-11
    Average 96 stars, based on 316 article reviews
    methylation sensitive restriction enzyme apeki - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum.
    Article Snippet: There were four treatment groups for each sample: uncut, methyl-sensitive enzyme (MSRE; Hha I or Hpa II; New England Biolabs, Ipswich, MA), methyl-dependent enzyme (McrBC; New England Biolabs, Ipswich, MA), and double digest with a methyl-sensitive and a methyl-dependent enzyme.

    Article Title: Cell-Wide DNA De-Methylation and Re-Methylation of Purkinje Neurons in the Developing Cerebellum
    Article Snippet: There were four treatment groups for each sample: uncut, methyl-sensitive enzyme (MSRE; Hha I or Hpa II; New England Biolabs, Ipswich, MA), methyl-dependent enzyme ( Mcr BC; New England Biolabs, Ipswich, MA), and double digest with a methyl-sensitive and a methyl-dependent enzyme.

    Article Title: Allelic 'choice' governs somatic hypermutation in vivo at the immunoglobulin kappa-chain locus.
    Article Snippet: Monoallelic demethylation and rearrangement control allelic exclusion of the immunoglobulin j-chain locus (Igk locus) in B cells.. Here, through the introduction of pre-rearranged Igk genes into their physiological position, the critical rearrangement step was bypassed, thereby generating mice producing B cells simultaneously expressing two different immunoglobulin-j light chains.. Such ‘double-expressing’ B cells still underwent monoallelic demethylation at the Igk locus, and the demethylated allele was the ‘preferred’ substrate for somatic hypermutation in each cell.



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